Analysis of somatic hybrids using non-radioactive hybridization probes.

Hybridization Capture Using RAD Probes

analysis of ruin probability for insurance companies using markov chain

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In situ hybridization with non-radioactive digoxigenin-11-UTP-labeled cRNA probes: localization of developmentally regulated mouse tenascin mRNAs.

An improved method for in situ hybridization was developed in order to identify the tissue-specific expression of messenger RNA (mRNA) for the novel extracellular matrix glycoprotein, tenascin, during mouse development. Non-radioactive RNA probes were generated by incorporating digoxigenin-11-UTP instead of conventional isotopic labels. Hybridization of anti-sense probes to complementary mRNAs ...

Increasing the specificity of colony hybridization when using heterologous probes.

Goat anti-mouse immunoglobulin horseradish peroxidase (HRP) conjugate was diluted in PBS containing 10% BSA. This conjugate has been successfully used from various manufacturers; however, specific lots need to be titrated for optimal performance. One particular preparation of HRPconjugated goat anti-mouse IgG, IgM and IgA (Catalog No. A 0412; Sigma Chemical, St. Louis, MO, USA) was diluted at 1...

Controlled Production of Proliferating Somatic Cell Hybrids

The techniques described permit the controlled production of large numbers of proliferating somatic cell hybrids in a relatively short period of time. Sendai virus is used to promote cell hybridization. beta-propriolactone is employed as the inactivating agent of Sendai virus since it produces complete loss of viral infectivity while preserving viral fusion capacity. Cells are fused in monolaye...